Transiently populated intermediate functions as a branching point of the FF domain folding pathway
نویسندگان
چکیده
منابع مشابه
Transiently populated intermediate functions as a branching point of the FF domain folding pathway.
Studies of protein folding and the intermediates that are formed along the folding pathway provide valuable insights into the process by which an unfolded ensemble forms a functional native conformation. However, because intermediates on folding pathways can serve as initiation points of aggregation (implicated in a number of diseases), their characterization assumes an even greater importance....
متن کاملCross-validation of the structure of a transiently formed and low populated FF domain folding intermediate determined by relaxation dispersion NMR and CS-Rosetta.
We have recently reported the atomic resolution structure of a low populated and transiently formed on-pathway folding intermediate of the FF domain from human HYPA/FBP11 [Korzhnev, D. M.; Religa, T. L.; Banachewicz, W.; Fersht, A. R.; Kay, L.E. Science 2011, 329, 1312-1316]. The structure was determined on the basis of backbone chemical shift and bond vector orientation restraints of the invis...
متن کاملDemonstration by burst-phase analysis of a robust folding intermediate in the FF domain.
The role of intermediates in the folding reaction of single-domain proteins is a controversial issue. It was previously shown by different methods that an on-pathway intermediate is populated in the presence of sodium sulphate during the folding of the FF domain from HYPA/FBP11. Here we demonstrate using analysis of the amplitudes of kinetic traces that this burst-phase folding intermediate is ...
متن کاملIs an intermediate state populated on the folding pathway of ubiquitin?
In the last couple of years, there has been increasing debate as to the presence and role of intermediate states on the folding pathways of several small proteins, including the 76-residue protein ubiquitin. Here, we present detailed kinetic studies to establish whether an intermediate state is ever populated during the folding of this protein. We show that the differences observed in previous ...
متن کاملThe Role of Aldehydes as Degenerate Branching Intermediate in the Oxidation of Hydrocarbons
The thermal oxidation of propane in the temperature range 350-425oC was studied in order to elucidate the role of higher aldehydes as degenerate branching intermediates in the oxidation of hydrocarbons. In the slow combustion of propane, the high yield of propylene and methanol as the primary products, the formation of hydrogen peroxide, carbon monoxide, carbon dioxide, formaldehyde and steam a...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 2012
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.1201799109